Valentin Dunsing
Biography
2021 - now Postdoc with PF. Lenne IBDM, Aix-Marseille University
2020 Postdoc with S. Chiantia, Cell Membrane Biophysics Group, University of Potsdam, Germany
2016-2020 PhD Thesis with S. Chiantia, Cell Membrane Biophysics Group, University of Potsdam, Germany
2012-2015 MSc Studies in Physics, Humboldt-University of Berlin, Germany and University of British Columbia, Vancouver, Canada
MSc Thesis with A. Herrmann and S. Chiantia, Molecular Biophysics Group, HU Berlin, Germany
Research Project with D. Coombs, Applied Mathematics Group, UBC, Vancouver, Canada2009-2012 BSc Studies in Physics, Humboldt-University of Berlin, Germany
BSc Thesis with A. Saez, Modern Optics Group, HU Berlin, Germany
Main research interest
Biological systems are highly dynamic. My research aims to understand how multicellular organization emerges from the dynamics and interactions of its constituents, on the molecular, cellular and tissue scale. To this aim, I apply and develop optical microscopy approaches such as 3D live imaging based on lightsheet microscopy and quantitative fluorescence techniques such as fluorescence fluctuation spectroscopy to observe molecular and cellular dynamics in living systems.
Current projects
...
Publications
Benchmarking of novel green fluorescent proteins for the quantification of protein oligomerization in living cells
Petrich, A., Koikkarah Aji, A., Dunsing, V., Chiantia, S., bioRxiv 2023.02.07.527542; doi: https://doi.org/10.1101/2023.02.07.527542Technical insights into fluorescence lifetime microscopy of mechanosensitive Flipper probes
Chloé Roffay, C., García-Arcos, JM., Chapuis, P., López-Andarias,J., Schneider, F., Colom, A., Tomba, C., Di Meglio, I., Dunsing, V., Matile, S., Roux, A., Mercier, V. (2022) bioRxiv 2022.09.28.509885; doi: https://doi.org/10.1101/2022.09.28.509885Biomimetic asymmetric bacterial membranes incorporating lipopolysaccharides
Stephan, M., Dunsing, V., Pramanik, S., Chiantia, S., Barbirz, S., Robinson, T., Dimova, R. (2022). Biophysical Journal, 2022 Dec 15;S0006-3495(22)03927-3. doi: 10.1016/j.bpj.2022.12.017.Mandipropamid as chemical inducer of proximity for in vivo application and protein network manipulation
Ziegler, M.J., Yserentant, K., Dunsing, V., Middel, V., Gralak, A.J., Pakari, K., Bargstedt, J., Kern, C., Petrich, A, Chiantia, S., Strähle, U., Herten, D-P., Wombacher, R. (2022). Nature Chemical Biology, 18(1), 64-69Influenza A M2 recruits M1 to the plasma membrane: a fluorescence fluctuation microscopy study
Petrich, A., Dunsing, V., Bobone, S., Chiantia, S. (2021). Biophysical Journal, Volume 120, Issue 4, Pages 5478-5490Multi-color fluorescence fluctuation spectroscopy in living cells via spectral detection
Dunsing, V.#, Petrich, A., Chiantia, S.# (2021). eLife 2021;10:e69687. #co-corresponding authorsPantoea stewartii WceF is a glycan biofilm modifying enzyme with a bacteriophage tailspike-like fold
Irmscher, T., Roske, Y., Gayk, I., Dunsing, V., Chiantia, S., Heinemann, U., Barbirz, S. (2021). Journal of Biological Chemistry 296 100286Purely polysaccharide-based biofilm matrix provides size-selective diffusion barriers for nanoparticles and bacteriophages
Dunsing, V., Irmscher, T., Barbirz, S., Chiantia, S. (2019). Biomacromolecules 20, 3842-3854.A fluorescence fluctuation spectroscopy assay of protein-protein interactions at cell-cell contacts
Dunsing, V., Chiantia, S. (2018). Journal of Visualized Experiments (142), e58582.Optimal fluorescent protein tags for quantifying protein oligomerization in living cells
Dunsing, V.#, Luckner, M.#, Zühlke, B., Petazzi, R.A., Herrmann, A., Chiantia, S. (2018). Scientific Reports 8:10634. #shared first authorsDirect evidence of amyloid precursor-like protein 1 trans interactions in cell-cell adhesion platforms investigated via fluorescence fluctuation spectroscopy
Dunsing, V., Mayer, M., Liebsch, F., Multhaup, G., Chiantia, S. (2017). Molecular Biology of the Cell 28, 3609-3620.Phosphatidylserine lateral organization influences the interaction of influenza virus matrix protein 1 with lipid membranes
Bobone, S., Hilsch, M., Storm, J., Dunsing, V., Herrmann, A., Chiantia, S. (2017). Journal of Virology 91:e00267-17.Amyloid precursor-like protein 1 (APLP1) exhibits stronger zinc-dependent neuronal adhesion than amyloid precursor protein and APLP2
Mayer, M. C., Schauenburg, L., Thompson-Steckel, G., Dunsing, V., Kaden, D., Voigt, P., Schaefer, M., Chiantia, S., Kennedy, T. E. and Multhaup, G. (2016). Journal of Neurochemistry, 137: 266-276.Design parameters for granzyme mediated cytotoxic lymphocyte target cell killing and specificity
Woodsworth, D., Dunsing, V., Coombs, D. (2015). Biophysical Journal, Volume 109, Issue 3, Pages 477-488