Biophysics of multicellular self-organization
Organoids and gastruloids
Among these self-organizing in vitro multicellular systems, Gastruloids are 3D cellular aggregates of embryonic stem cells capable of differentiating and organizing in a manner reminiscent of the gastrulation process in the embryo. Cultured over several days in specific culture mediums, Gastruloids can develop advanced structures strikingly similar to organs such as (1) somites and neural-tube or (2) gut and heart. The first days of self-organization of these organoids have identifiable commonalities among these different classes of chemical protocols leading to the formation of different organ-like structures.
3D imaging of embyronic organoids
Gastruloids biophysical mechanisms of symmetry breaking
Understanding how a spherical aggregate of apparently homogeneous cells self-organize itself into a polarized structure with a recognizable axis is a fundamental question that has implications for the formation of the primary body axis during embryonic development, as well as the development of most, if not all, organoids. To address this fundamental question, we investigate the behavior of individual cells within the aggregate, such as cell movement, division, and death. We identify mechanisms of symmetry breaking including tissue flows and cell differentiation.
mechanical stresses and rheological properties of SELF-ORGANAZING MULTICELLULAR SYSTEMS
Collaborations: Léo Guignard (LIS, CENTURI, Marseille), Simon Gsell (IRPHE, Marseille), Alfonso Martinez Arias (Universidad Pompeu Fabra, Barcelona), Matthias Merkel (CPT, CENTURI, Marseille), Philippe Roudot (I2M, CENTURI, Marseille), Vikas Trivedi (EMBL, Barcelona), Virgile Viasnoff (CINAM, Marseille)
Funding: Fondation de la Recherche Médicale (FRM), ANR, CENTURI, ERC