Ressources
In VIVO OPTICAL Manipulation
Trapping and manipulation of cell-cell contacts
Since our first paper (Bambardekar et al. 2015), we have published two methodological papers with detailed protocols:
Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo. Chardès, C., Clément, R., Blanc, O., and Lenne, P.-F. (2018). J Vis Exp, e57900. 10.3791/57900.
Two-Point Optical Manipulation of Cell Junctions in the Early Epithelium of the Drosophila Embryo. Nishizawa, K., Chardès, C., Clément, R., and Lenne, P.-F. (2023). Methods Mol Biol 2600, 107–118. 10.1007/978-1-0716-2851-5_7.
Laser nanodissection
Our technique uses a near-infrared femtosecond laser to break sub-micrometer sized structures with little collateral damage, e.g. we can break the cell cortex without damaging the neighboring cell membranes.
See our method papers:
Laser Ablation to Probe the Epithelial Mechanics in Drosophila. Shivakumar, P.C., and Lenne, P.-F. (2016). Methods Mol. Biol. 1478, 241–251. 10.1007/978-1-4939-6371-3_14.
Probing cell mechanics with subcellular laser dissection of actomyosin networks in the early developing Drosophila embryo. Rauzi, M., and Lenne, P.F. (2015). Methods Mol. Biol. 1189, 209–218. 10.1007/978-1-4939-1164-6_14.