Lenne group - Publications

Publications

2023

Lenne, P.-F., and Tlili, S. (2023). Learning the mechanobiology of development from gastruloids. Emerg Top Life Sci 7, 417–422. 10.1042/ETLS20230081.

Gsell, S., Tlili, S., Merkel, M., and Lenne, P.-F. (2023). Marangoni-like tissue flows enhance symmetry breaking of embryonic organoids. Preprint at bioRxiv, 10.1101/2023.09.22.559003 10.1101/2023.09.22.559003.

Nishizawa, K., Lin, S.-Z., Chardès, C., Rupprecht, J.-F., and Lenne, P.-F. (2023). Two-point optical manipulation reveals mechanosensitive remodeling of cell–cell contacts in vivo. Proceedings of the National Academy of Sciences 120, e2212389120. 10.1073/pnas.2212389120.

Vanaret, J., Dupuis, V., Lenne, P.-F., Richard, F.-J.-P., Tlili, S. and Roudot, P. (2023). A detector-independent quality score for cell segmentation without ground truth in 3D live fluorescence microscopy. IEEE Journal of Selected Topics in Quantum Electronics. hal-03923509.

Recouvreux, P., Pai, P., Torro, R., Ludányi, M., Mélénec, P., Boughzala, M., Bertrand, V., and Lenne, P.-F. (2023). Establishment of Wnt ligand-receptor organization and cell polarity in the C. elegans embryo. BioRxiv, 2023.01.17.524363. 10.1101/2023.01.17.524363.

Nishizawa, K., Chardès, C., Clément, R., and Lenne, P.-F. (2023). Two-Point Optical Manipulation of Cell Junctions in the Early Epithelium of the Drosophila Embryo. Methods Mol Biol 2600, 107–118. 10.1007/978-1-0716-2851-5_7.

Fu, C., Dilasser F., Lin, S-Z., Karnat, M., Arora, A., Rajendiran, H., Ong, H-T., Hoon, N-M., Phow, S-W., Hirashima, T., Sheezt, M., Rupprecht, J-F., Tlili, S., Viasnoff , V. (2023). E-cadherin-dependent phosphorylation of EGFR governs a homeostatic feedback loop controlling intercellular junction

viscosity and collective migration modes. BioRxiv, 2023.12. 10.1101/2023.12.04.570034.

2022

Mao, Q., Acharya, A., Rodríguez-delaRosa, A., Marchiano, F., Dehapiot, B., Al Tanoury, Z., Rao, J., Díaz-Cuadros, M., Mansur, A., Wagner, E., et al. (2022). Tension-driven multi-scale self-organisation in human iPSC-derived muscle fibers. eLife 11, e76649. 10.7554/eLife.76649.

Mangeol, P., Massey-Harroche, D., Richard, F., Concordet, J.-P., Lenne, P.-F., and Le Bivic, A. (2022). Super-resolution imaging uncovers the nanoscopic segregation of polarity proteins in epithelia. Elife 11, e62087. 10.7554/eLife.62087.

Tlili, S. (2022). Unjammed development. Nature Physics 18, 1400. 10.1038/s41567-022-01796-5

Tlili, S.L., Graner, F. and Delanoë-Ayari, H. (2022). A microfluidic platform to investigate the role of mechanical constraints on tissue reorganization. Development; 149 (20): dev200774. 10.1242/dev.200774

Lenne, P.-F., and Trivedi, V. (2022). Sculpting tissues by phase transitions. Nature Communications 13, 664. 10.1038/s41467-022-28151-9.

Hashmi, A., Tlili, S., Perrin, P., Lowndes, M., Peradziryi, H., Brickman, J.M., Martínez Arias, A., and Lenne, P.-F. (2022). Cell-state transitions and collective cell movement generate an endoderm-like region in gastruloids. Elife 11, e59371. 10.7554/eLife.59371.

2021

Veenvliet, J.V., Lenne, P.-F., Turner, D.A., Nachman, I., and Trivedi, V. (2021). Sculpting with stem cells: how models of embryo development take shape. Development 148, dev192914. 10.1242/dev.192914.

Lenne, P.-F., Rupprecht, J.-F., and Viasnoff, V. (2021). Cell Junction Mechanics beyond the Bounds of Adhesion and Tension. Developmental Cell 0. 10.1016/j.devcel.2020.12.018.

Lenne, P.-F., Munro, E., Heemskerk, I., Warmflash, A., Bocanegra-Moreno, L., Kishi, K., Kicheva, A., Long, Y., Fruleux, A., Boudaoud, A., et al. (2021). Roadmap for the multiscale coupling of biochemical and mechanical signals during development. Phys. Biol. 18, 041501. 10.1088/1478-3975/abd0db.

2020

Kaur, S., Mélénec, P., Murgan, S., Bordet, G., Recouvreux, P., Lenne, P.-F., and Bertrand, V. (2020). Wnt ligands regulate the asymmetric divisions of neuronal progenitors in C. elegans embryos. Development 147. 10.1242/dev.183186.

2019

Mangeol, P., Massey-Harroche, D., Bivic, A.L., and Lenne, P.-F. (2019). Nectins rather than E-cadherin anchor the actin belts at cell-cell junctions of epithelia. bioRxiv, 809343.

Kong, W., Loison, O., Chavadimane Shivakumar, P., Chan, E.H., Saadaoui, M., Collinet, C., Lenne, P.-F., and Clément, R. (2019). Experimental validation of force inference in epithelia from cell to tissue scale. Sci Rep 9, 14647. 10.1038/s41598-019-50690-3.

Bailles, A., Collinet, C., Philippe, J.-M., Lenne, P.-F., Munro, E., and Lecuit, T. (2019). Genetic induction and mechanochemical propagation of a morphogenetic wave. Nature 572, 467–473. 10.1038/s41586-019-1492-9.

2018

Toret, C.P., Shivakumar, P.C., Lenne, P.-F., and Le Bivic, A. (2018). The elmo-mbc complex and rhogap19d couple Rho family GTPases during mesenchymal-to-epithelial-like transitions. Development (Cambridge, England) 145, dev157495. 10.1242/dev.157495.

Loison, O., Weitkunat, M., Kaya-Çopur, A., Nascimento Alves, C., Matzat, T., Spletter, M.L., Luschnig, S., Brasselet, S., Lenne, P.-F., and Schnorrer, F. (2018). Polarization-resolved microscopy reveals a muscle myosin motor-independent mechanism of molecular actin ordering during sarcomere maturation. PLoS Biol 16, e2004718. 10.1371/journal.pbio.2004718.

Lenne, P.-F., and Trivedi, V. (2018). Tissue ‘melting’ sculpts embryo. Nature 561, 315–316. 10.1038/d41586-018-06108-7.

Kong, W., Loison, O., Chavadimane Shivakumar, P., Collinet, C., Lenne, P.-F., and Clément, R. (2018). Force inference predicts local and tissue-scale stress patterns in epithelia. bioRxiv, 475012. 10.1101/475012.

Kale, G.R., Yang, X., Philippe, J.-M., Mani, M., Lenne, P.-F., and Lecuit, T. (2018). Distinct contributions of tensile and shear stress on E-cadherin levels during morphogenesis. Nature Communications 9, 5021. 10.1038/s41467-018-07448-8.

Chardès, C., Clément, R., Blanc, O., and Lenne, P.-F. (2018). Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo. J Vis Exp, e57900. 10.3791/57900.

2017

Clément, R., Dehapiot, B., Collinet, C., Lecuit, T., and Lenne, P.-F. (2017). Viscoelastic Dissipation Stabilizes Cell Shape Changes during Tissue Morphogenesis. Curr Biol 27, 3132-3142.e4. 10.1016/j.cub.2017.09.005.

Chan, E.H., Chavadimane Shivakumar, P., Clément, R., Laugier, E., and Lenne, P.-F. (2017). Patterned cortical tension mediated by N-cadherin controls cell geometric order in the Drosophila eye. eLife 6, e22796. 10.7554/eLife.22796.

Before 2017

Sugimura, K., Lenne, P.-F., and Graner, F. (2016). Measuring forces and stresses in situ in living tissues. Development 143, 186–196. 10.1242/dev.119776.

Shivakumar, P.C., and Lenne, P.-F. (2016). Laser Ablation to Probe the Epithelial Mechanics in Drosophila. Methods Mol. Biol. 1478, 241–251. 10.1007/978-1-4939-6371-3_14.

Recouvreux, P., and Lenne, P.-F. (2016). Molecular clustering in the cell: from weak interactions to optimized functional architectures. Current opinion in cell biology 38, 18–23. 10.1016/j.ceb.2016.01.002.

Kim, E., Felouat, A., Zaborova, E., Ribierre, J.-C., Wu, J.W., Sénatore, S., Matthews, C., Lenne, P.-F., Baffert, C., Karapetyan, A., et al. (2016). Borondifluoride complexes of hemicurcuminoids as bio-inspired push-pull dyes for bioimaging. Org. Biomol. Chem. 14, 1311–1324. 10.1039/c5ob02295a.

Kamada, K., Namikawa, T., Sénatore, S., Matthews, C., Lenne, P.-F., Maury, O., Andraud, C., Ponce-Vargas, M., Le Guennic, B., Jacquemin, D., et al. (2016). Boron Difluoride Curcuminoid Fluorophores with Enhanced Two-Photon Excited Fluorescence Emission and Versatile Living-Cell Imaging Properties. Chemistry 22, 5219–5232. 10.1002/chem.201504903.

Truong Quang, B.-A., and Lenne, P.-F. (2015). Superresolution measurements in vivo: imaging Drosophila embryo by photoactivated localization microscopy. Methods Cell Biol. 125, 119–142. 10.1016/bs.mcb.2014.10.015.

Rauzi, M., and Lenne, P.F. (2015). Probing cell mechanics with subcellular laser dissection of actomyosin networks in the early developing Drosophila embryo. Methods Mol. Biol. 1189, 209–218. 10.1007/978-1-4939-1164-6_14.

Markova, O., Sénatore, S., Chardès, C., and Lenne, P.-F. (2015). Calcium Spikes in Epithelium: study on Drosophila early embryos. Scientific Reports 5, 11379. 10.1038/srep11379.

Collinet, C., Rauzi, M., Lenne, P.-F., and Lecuit, T. (2015). Local and tissue-scale forces drive oriented junction growth during tissue extension. Nature Cell Biology 17, 1247–1258. 10.1038/ncb3226.

Bambardekar, K., Clément, R., Blanc, O., Chardès, C., and Lenne, P.-F. (2015). Direct laser manipulation reveals the mechanics of cell contacts in vivo. Proceedings of the National Academy of Sciences of the United States of America. 10.1073/pnas.1418732112.

Truong Quang, B.-A., and Lenne, P.-F. (2014). Membrane microdomains: from seeing to understanding. Front Plant Sci 5, 18. 10.3389/fpls.2014.00018.

Markova, O., Alberts, J., Munro, E., and Lenne, P.-F. (2014). Clustering of low-valence particles: structure and kinetics. Phys Rev E Stat Nonlin Soft Matter Phys 90, 022301. 10.1103/PhysRevE.90.022301.

Chardès, C., Mélénec, P., Bertrand, V., and Lenne, P.-F. (2014). Setting up a simple light sheet microscope for in toto imaging of C. elegans development. J Vis Exp. 10.3791/51342.

Quang, B., Mani, M., Markova, O., Lecuit, T., and Lenne, P. (2013). Principles of E-Cadherin Supramolecular Organization In Vivo. CURRENT BIOLOGY 23, 2197–2207. 10.1016/j.cub.2013.09.015.

Markova, O., Alberts, J., Munro, E., and Lenne, P.F. (2012). Bond flexibility and low valence promote finite clusters of self-aggregating particles. Phys. Rev. Lett. 109, 078101.

Rauzi, M., and Lenne, P.-F. (2011). Cortical forces in cell shape changes and tissue morphogenesis. Curr. Top. Dev. Biol. 95, 93–144. 10.1016/B978-0-12-385065-2.00004-9.

Lecuit, T., Lenne, P.-F., and Munro, E. (2011). Force generation, transmission, and integration during cell and tissue morphogenesis. Annu. Rev. Cell Dev. Biol. 27, 157–184. 10.1146/annurev-cellbio-100109-104027.

Rauzi, M., Lenne, P.F., and Lecuit, T. (2010). Planar polarized actomyosin contractile flows control epithelial junction remodelling. Nature 468, 1110–1114. 10.1038/nature09566.

Lenne, P.-F. (2009). Probing cell-surface dynamics and mechanics at different scales. Histochem. Cell Biol. 132, 247–252. 10.1007/s00418-009-0620-2.

Rauzi, M., Verant, P., Lecuit, T., and Lenne, P.-F. (2008). Nature and anisotropy of cortical forces orienting Drosophila tissue morphogenesis. Nature Cell biology 10, 1401–1410. 10.1038/ncb1798.

Lenne, P.-F., Rigneault, H., Marguet, D., and Wenger, J. (2008). Fluorescence fluctuations analysis in nanoapertures: physical concepts and biological applications. Histochem. Cell Biol. 130, 795–805. 10.1007/s00418-008-0507-7.

Lasserre, R., Guo, X.-J., Conchonaud, F., Hamon, Y., Hawchar, O., Bernard, A.-M., Soudja, S.M., Lenne, P.-F., Rigneault, H., Olive, D., et al. (2008). Raft nanodomains contribute to Akt/PKB plasma membrane recruitment and activation. 4, 538–547. 10.1038/nchembio.103.

Cavey, M., Rauzi, M., Lenne, P.-F., and Lecuit, T. (2008). A two-tiered mechanism for stabilization and immobilization of E-cadherin. Nature 453, 751–756. 10.1038/nature06953.

Wenger, J., Conchonaud, F., Dintinger, J., Wawrezinieck, L., Ebbesen, T.W., Rigneault, H., Marguet, D., and Lenne, P.-F. (2007). Diffusion analysis within single nanometric apertures reveals the ultrafine cell membrane organization. Biophys. J. 92, 913–919. 10.1529/biophysj.106.096586.

Wenger, J., Cluzel, B., Dintinger, J., Bonod, N., Fehrembach, A.-L., Popov, E., Lenne, P.-F., Ebbesen, T.W., and Rigneault, H. (2007). Radiative and non-radiative photokinetics alteration inside a single metallic nanometric aperture.

Popov, E., Nevière, M., Sentenac, A., Bonod, N., Fehrembach, A.-L., Wenger, J., Lenne, P.-F., and Rigneault, H. (2007). Single-scattering theory of light diffraction by a circular subwavelength aperture in a finitely conducting screen. J Opt Soc Am A Opt Image Sci Vis 24, 339–358.

Lecuit, T., and Lenne, P.-F. (2007). Cell surface mechanics and the control of cell shape, tissue patterns and morphogenesis. Nature Reviews Molecular Cell Biology 8, 633–644. 10.1038/nrm2222.